Perform calculations for large scale PCR reactions and ensure success of scaled up reactions with this online PCR Master Mix Calculator and you can use the oligonucleotide properties calculator. Use checkboxes to remermer added componets.
Equations assume that the annealing occurs under the standard conditions of 50 nM primer, 50 mM Na+, and pH 7.0.
Tm= 64.9 +41*(yG+zC-16.4)/(wA+xT+yG+zC)
Equations assume that the annealing occurs under the standard conditions of 50 nM primer, 50 mM Na+, and pH 7.0.
Tm= 64.9 +41*(yG+zC-16.4)/(wA+xT+yG+zC)
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