1. OBJECTIVE
In this experiment, you will learn about the different nutritional needs of microorganisms. Additionally, the experiment develops the ability to prepare the culture media used in the clinical laboratory, from weighing to final storage.
At the end of this experiment, you should be able to:
Prepare culture media;
carry out quality control of the culture media;
identify alterations in the culture media;
learn to report the visualized result of quality controls.
2. Where to use these concepts?
Knowing how to prepare and carry out quality control of culture media is a prerequisite for the development of experimental skills and abilities that enable the diagnosis of bacterial and fungal infections. In addition, the use of an appropriate culture medium makes it possible to identify the etiologic agent.
3. The experiment
This experiment will use the following inputs: bench disinfection kit (almotolia with alcohol and hypochlorite), dehydrated media and distilled or deionized water and instruments that will serve to assist in the execution of the practice, such as calibrated scales, glassware (test tube and Erlenmeyer), spatulas and sealing materials.
4. Security
In this practice, gloves, mask and lab coat, also called dust guard, will be used. Although the practice does not pose a risk to the student, these three protective equipment are essential for the laboratory environment. Gloves will prevent possible cuts or contamination with agents harmful to the skin. The mask protects against possible aerosols and the lab coat protects the body as a whole.
5. Scenario
The experiment environment features a Bunsen burner on top of the workbench, as well as inputs and instruments. You must select and use them in order to guarantee the correct execution of the experiments. In addition, after making the medium, it must be autoclaved and taken to the laminar flow hood.
In this experiment, you will learn about the different nutritional needs of microorganisms. Additionally, the experiment develops the ability to prepare the culture media used in the clinical laboratory, from weighing to final storage.
At the end of this experiment, you should be able to:
Prepare culture media;
carry out quality control of the culture media;
identify alterations in the culture media;
learn to report the visualized result of quality controls.
2. Where to use these concepts?
Knowing how to prepare and carry out quality control of culture media is a prerequisite for the development of experimental skills and abilities that enable the diagnosis of bacterial and fungal infections. In addition, the use of an appropriate culture medium makes it possible to identify the etiologic agent.
3. The experiment
This experiment will use the following inputs: bench disinfection kit (almotolia with alcohol and hypochlorite), dehydrated media and distilled or deionized water and instruments that will serve to assist in the execution of the practice, such as calibrated scales, glassware (test tube and Erlenmeyer), spatulas and sealing materials.
4. Security
In this practice, gloves, mask and lab coat, also called dust guard, will be used. Although the practice does not pose a risk to the student, these three protective equipment are essential for the laboratory environment. Gloves will prevent possible cuts or contamination with agents harmful to the skin. The mask protects against possible aerosols and the lab coat protects the body as a whole.
5. Scenario
The experiment environment features a Bunsen burner on top of the workbench, as well as inputs and instruments. You must select and use them in order to guarantee the correct execution of the experiments. In addition, after making the medium, it must be autoclaved and taken to the laminar flow hood.
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