1. OBJECTIVE
This technique allows staining by the fast panoptic method of a thin layer of fresh blood on a glass slide (hematologic distention) for subsequent observation under an optical microscope.
At the end of this experiment, you should be able to:
correctly stain a haematological distention;
correctly use instruments and dyes for hematological distension;
recognize the step in which any error occurred in the staining.
2. WHERE TO USE THESE CONCEPTS?
Perform manual staining of hematological distention for subsequent qualitative and quantitative analysis of cells circulating in the bloodstream. The staining of blood distension is one of the main steps that precede the blood count. This exam aims to investigate diseases directly related to hematological cells, or clinical conditions that induce morphological or quantitative changes in cells that make up the immune system, such as infections and inflammations.
3. THE EXPERIMENT
This experiment consists of manual staining of the hematological distension for subsequent qualitative and quantitative analysis of the cells circulating in the bloodstream. For this, the following supplies will be needed: microscopy slide with dry hematological distention, panoptic staining kit, Coplin or Wertheim vats (glass cuvettes for dyes), deionized water (pH 7.0), paper towel and optical microscope .
4. SECURITY
In this practice, for the staining procedure, procedure gloves, lab coat, protective glasses and disposable masks will be used. Although the practice does not pose a risk to the student, these personal protective equipment (PPE) are essential for the laboratory environment. The glove will prevent any possible cut or contamination with blood and the lab coat protects from blood splashes on the body. The goggles and mask will protect against splashing blood or reagents in the eyes and mouth.
5. SCENARIO
The experiment environment has a microscopy slide with a sample/dried blood smear and sample identification on the matte part of the slide; bench with four glass cuvettes (also known as Coplin or Wertheim vats), measuring 90 mm (length) by 35 mm (height), for dyes 1, 2 and 3 (panoptic staining kit), the fourth cuvette for deionized water ; paper towel; procedure gloves; coat; optical microscope. You must select and use them in order to guarantee the correct execution of the experiments.
This technique allows staining by the fast panoptic method of a thin layer of fresh blood on a glass slide (hematologic distention) for subsequent observation under an optical microscope.
At the end of this experiment, you should be able to:
correctly stain a haematological distention;
correctly use instruments and dyes for hematological distension;
recognize the step in which any error occurred in the staining.
2. WHERE TO USE THESE CONCEPTS?
Perform manual staining of hematological distention for subsequent qualitative and quantitative analysis of cells circulating in the bloodstream. The staining of blood distension is one of the main steps that precede the blood count. This exam aims to investigate diseases directly related to hematological cells, or clinical conditions that induce morphological or quantitative changes in cells that make up the immune system, such as infections and inflammations.
3. THE EXPERIMENT
This experiment consists of manual staining of the hematological distension for subsequent qualitative and quantitative analysis of the cells circulating in the bloodstream. For this, the following supplies will be needed: microscopy slide with dry hematological distention, panoptic staining kit, Coplin or Wertheim vats (glass cuvettes for dyes), deionized water (pH 7.0), paper towel and optical microscope .
4. SECURITY
In this practice, for the staining procedure, procedure gloves, lab coat, protective glasses and disposable masks will be used. Although the practice does not pose a risk to the student, these personal protective equipment (PPE) are essential for the laboratory environment. The glove will prevent any possible cut or contamination with blood and the lab coat protects from blood splashes on the body. The goggles and mask will protect against splashing blood or reagents in the eyes and mouth.
5. SCENARIO
The experiment environment has a microscopy slide with a sample/dried blood smear and sample identification on the matte part of the slide; bench with four glass cuvettes (also known as Coplin or Wertheim vats), measuring 90 mm (length) by 35 mm (height), for dyes 1, 2 and 3 (panoptic staining kit), the fourth cuvette for deionized water ; paper towel; procedure gloves; coat; optical microscope. You must select and use them in order to guarantee the correct execution of the experiments.
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