1. OBJECTIVE
This technique allows the determination of the number of erythrocytes per mm3 (erythrocyte concentration) of a whole blood sample using a Neubauer chamber.
At the end of this experiment, you should be able to:
Determine the number of erythrocytes in mm3 of a whole blood sample using the Neubauer chamber;
perform the sample dilution correctly;
identify the place in the Neubauer chamber where the erythrocyte count should be performed;
correctly perform the calculations for converting the erythrocyte count per field into the number of erythrocytes per mm3;
correctly use the Neubauer chamber for erythrocyte counting;
recognize the error in execution, if any, that gave rise to the inadequate result.
2. WHERE TO USE THESE CONCEPTS?
Manual counting of erythrocytes is necessary to confirm the result obtained by the automated counting device, because, in some serious clinical conditions, such as specific leukemias, the automation device does not correctly detect the formed elements of blood, such as erythrocytes. Thus, counting using the Neubauer chamber is recommended to prove or disprove the results obtained through automated counting.
3. THE EXPERIMENT
This experiment consists of determining the number of erythrocytes in a whole blood sample using a Neubauer chamber. For this, several inputs will be used, such as: whole blood sample collected in a 13x75mm collection tube, with a 2mL aspiration capacity, containing 15% EDTA k2 anticoagulant; 5mL test tube; Gower's reagent or liquid; Neubauer chamber; micropipette (fixed volume) with a capacity of 1,000µL and its respective tip; micropipette (fixed volume) with a capacity of 20µL and its respective tip; gauze; optical microscope.
4. SECURITY
In this practice, procedure gloves, lab coat and goggles will be used. Although the practice generates little risk for the student, these protective equipment are essential for the laboratory environment. The glove will prevent contamination with blood if your hand gets dirty, and the lab coat protects against blood splashes on the body or clothing. The use of protective goggles prevents splashes of the blood sample from coming into contact with the eyes.
5. SCENARIO
The experiment environment has a laboratory bench, Neubauer chamber and its respective coverslip, test tubes in support, micropipettes with volumes from 20 to 1,000µL and their respective tips (yellow and blue), gauze and optical microscope. You must select and use them in order to guarantee the correct execution of the experiments.
This technique allows the determination of the number of erythrocytes per mm3 (erythrocyte concentration) of a whole blood sample using a Neubauer chamber.
At the end of this experiment, you should be able to:
Determine the number of erythrocytes in mm3 of a whole blood sample using the Neubauer chamber;
perform the sample dilution correctly;
identify the place in the Neubauer chamber where the erythrocyte count should be performed;
correctly perform the calculations for converting the erythrocyte count per field into the number of erythrocytes per mm3;
correctly use the Neubauer chamber for erythrocyte counting;
recognize the error in execution, if any, that gave rise to the inadequate result.
2. WHERE TO USE THESE CONCEPTS?
Manual counting of erythrocytes is necessary to confirm the result obtained by the automated counting device, because, in some serious clinical conditions, such as specific leukemias, the automation device does not correctly detect the formed elements of blood, such as erythrocytes. Thus, counting using the Neubauer chamber is recommended to prove or disprove the results obtained through automated counting.
3. THE EXPERIMENT
This experiment consists of determining the number of erythrocytes in a whole blood sample using a Neubauer chamber. For this, several inputs will be used, such as: whole blood sample collected in a 13x75mm collection tube, with a 2mL aspiration capacity, containing 15% EDTA k2 anticoagulant; 5mL test tube; Gower's reagent or liquid; Neubauer chamber; micropipette (fixed volume) with a capacity of 1,000µL and its respective tip; micropipette (fixed volume) with a capacity of 20µL and its respective tip; gauze; optical microscope.
4. SECURITY
In this practice, procedure gloves, lab coat and goggles will be used. Although the practice generates little risk for the student, these protective equipment are essential for the laboratory environment. The glove will prevent contamination with blood if your hand gets dirty, and the lab coat protects against blood splashes on the body or clothing. The use of protective goggles prevents splashes of the blood sample from coming into contact with the eyes.
5. SCENARIO
The experiment environment has a laboratory bench, Neubauer chamber and its respective coverslip, test tubes in support, micropipettes with volumes from 20 to 1,000µL and their respective tips (yellow and blue), gauze and optical microscope. You must select and use them in order to guarantee the correct execution of the experiments.
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